Application

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TICAM1 in human liver tissue.
Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected TICAM1 in HeLa and A431 cells.

Research Sub CategoryApoptosis - Additional

Anti-TICAM1 Antibody, clone 3D5.1 is an antibody against TICAM1 for use in Western Blotting, Immunohistochemistry, Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TIR domain-containing adapter molecule 1 (UniProt Q8IUC6), also known as MyD88-3, Proline-rich, vinculin and TIR domain-containing protein B, Putative NF-kB-activating protein 502H, TICAM-1, TIR domain containing adaptor inducing interferon-b, TIR domain-containing adapter protein inducing IFN-b, Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon b) is encoded by the TICAM1 gene in human. TCAM-1/TRIF/MyD88-3 is as an adaptor molecule that mediates Toll-like receptor 3 (TLR3)-dependent NF-kB activation and cytokine expression. Upon recognition of double-stranded RNA, TLR3 recruits TRIF, which in turn associates with TNF receptor-associated factor 3 (TRAF3) to activate two Ik-B kinase (IKK)-related kinases, TANK-binding kinase 1 (TBK1) and IKKi. These two kinases phosphorylate interferon regulatory factor 3/7 (IRF3/7), resulting in the induction of type I interferons (IFNs) and expression of IFN-inducible genes. TICAM-1 can also mediate NF-kB activation through receptor-interacting protein 1 (RIP1) and TRAF6. Human enterovirus 68 (EV68) is reported to disable TLR3 pathway signaling by cleaving TRIF in infected cells.

Immunogen

GST-tagged recombinant protein corresponding to human TICAM1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TICAM1 in 10 µg of Raji cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~76 kDa observed